Fig 2: Western blot results for the expression of molecules in the JAK-STAT3 pathway. A, C, E. LIF, STAT3, JAK proteins were expressed in ECA109 and KYSE150 cells. B, D, F. LIF, STAT3, JAK proteins expression following transfection with siLIF/carbon ion combination irradiation in ECA109 and KYSE150 cells.

Fig 3: Expression validation of LIF using single molecule in databases and tissues samples. A. The expression differences of LIF between ESCA patients and normal controls were conducted by GEPIA dataset. B. HE staining and IHC staining of LIF in ESCC tumor and normal tissues. C. The LIF staining scores for the ESCC tissue samples were higher than those for their adjacent normal tissue samples.

Fig 4: Effects of Aanat KO on endometrial receptivity and embryo implantation at 4.5 dpc in mice. (A,B) Implantation sites at 4.5 dpc, implantation window. As the red arrows indicate, the blue is the attachment sites displayed after Chicago Sky Blue staining. (C) Immunohistochemical staining of a pregnant uterus at 4.5 dpc; the brown staining indicates AANAT, and the blue staining indicates the nucleus. (D) Western blotting of BMP2 with statistical analysis. (E) HE staining of 4.5 dpc uteri. (F). Western blotting of the FOXA2 LIF protein with statistical analysis. (G) Immunohistochemical staining of a 4.5 dpc uterus; the brown signal is ERa and PR, and the blue signal is the nucleus. (H) Western blotting of the ERa protein with statistical analysis. (I) Western blotting of the PR-A protein with statistical analysis. (J) Expression of the downstream response genes of estrogen and progesterone by PCR, n = 6 Note: * p < 0.05, ** p < 0.01.